| | E-cadherin gene polymorphisms and susceptibility to urolithiasis in Iraqi children| العنوان باللغة العربية : | E كادهرين تعدد الأشكال الجينية وقابلية التحص البولي في الأطفال العراقيين | | العنوان باللغة الانكليزية : | E-cadherin gene polymorphisms and susceptibility to urolithiasis in Iraqi children | | المؤلف : | SHAHA HUSSEIN ALI | | البريد الالكتروني : | Shatha6ali@yahoo.com | | جهة انتساب المؤلف : | Department of Pediatrics, College of Medicine, Al Nahrain University, Al – Kadhymia, P. O. Box 70074, Baghdad, Iraq | | المشاركين : | HAIDAR AHMED SHAMRAN, MAYASA ABDULLA ALI, QASIM SHARHAN AL-MAYAH, ELHAM AHMED JASIM | | المستفيدين : | Iraq childrein | | المستخلص : | ABSTRACT: Aim: This case/control study aimed to assess the impact of two single nucleotide polymorphisms (SNPs) in the promoter region of CDH1 gene (-160C>A and -347G>GA) on urinary stone formation in a sample of Iraqi children. Methods: Forty-seven children with urolithiasis and 50 age- and gendermatched healthy controls were included in the study. DNA was isolated from peripheral blood and direct sequencing was used for genotyping. Results: The homozygous genotype of the SNP CHD1 -160C>A was more frequent in control than cases (18% vs. 6.38%) with significant difference (OR = 0.229, 95%CI = 0.056–0.943, P = 0.041). Furthermore, cases showed significantly less frequency of the mutant allele (allele A) of this SNP (OR = 0.403, 95%C = 0.210–0.776, P = 0.007). Conclusion: These results strongly indicate a protective role of allele A of the SNP CHD1 -160C>A against urinary calculi formation in children ABSTRACT: Aim: This case/control study aimed to assess the impact of two single nucleotide polymorphisms (SNPs) in the promoter region of CDH1 gene (-160C>A and -347G>GA) on urinary stone formation in a sample of Iraqi children. Methods: Forty-seven children with urolithiasis and 50 age- and gendermatched healthy controls were included in the study. DNA was isolated from peripheral blood and direct sequencing was used for genotyping. Results: The homozygous genotype of the SNP CHD1 -160C>A was more frequent in control than cases (18% vs. 6.38%) with significant difference (OR = 0.229, 95%CI = 0.056–0.943, P = 0.041). Furthermore, cases showed significantly less frequency of the mutant allele (allele A) of this SNP (OR = 0.403, 95%C = 0.210–0.776, P = 0.007). Conclusion: These results strongly indicate a protective role of allele A of the SNP CHD1 -160C>A against urinary calculi formation in children | | المستخلص باللغة العربية : | الهدف: تهدف دراسة الحالة / السيطرة هذه إلى تقييم تأثير تعدد الأشكال النوكليوتيدات الفردية في منطقة المروج لجين CDH1 (-160 درجة مئوية> A و -347 G> GA) على تكوين حصوات البول في عينة من الأطفال العراقيين. الطريقة: تم تضمين سبعة وأربعين طفلا مصاب بالتهاب المسالك البولية و 50 سنه والضوابط الصحية في الدراسة. تم عزل الحمض النووي من الدم المحيطي وكان التسلسل المباشر يستخدم للتنميط الجيني. النتائج: كان النمط الوراثي متماثل الزيجوت لـ SNP CHD1 -160C> A أكثر تواترًا في السيطرة من الحالات (18٪ مقابل 6.38٪) مع اختلاف كبير (OR = 0.229 ، 95٪ CI = 0.056–0.943 ، P = 0.041). علاوة على ذلك ، أظهرت الحالات انخفاضًا ملحوظًا في تواتر أليل متحولة (أليل A) لهذا SNP (OR = 0.403 ، 95٪ C = 0.210-0.776 ، P = 0.007). الخلاصة: هذه النتائج تشير بقوة إلى دور وقائي للأليل A من SNP CHD1 -160C> A ضد تشكيل الحصوات البولية في الأطفال | | كلمات مفتاحية : | E-cadherin, gene polymorphism, urolithiasis | | الرابط : | 10.1111/nep.13184 |
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| | | Genotyping of Cytomegalovirus from Symptomatic Infected Neonates in Iraq| العنوان باللغة العربية : | التصنيف الجيني للفيروس الخلوي المتضخم عند المواليد المصابين بالفايروس ولديهم اعراض. | | العنوان باللغة الانكليزية : | Genotyping of Cytomegalovirus from Symptomatic Infected Neonates in Iraq | | المؤلف : | Sevan N. Alwan | | البريد الالكتروني : | alobaidis@uthscsa.edu | | جهة انتساب المؤلف : | Department of Biochemistry and Structural Biology, UT Health at San Antonio, San Antonio, Texas | | المشاركين : | Haider S. Kadhim, Atheer J. AL-Saffar, Hala S. Arif, Jianmin Fu, and Brian L. Wickes, Haidar A. Shamran, Avan H. Ghaib, Qasim S. Al-Mayah, and Ali H. Bayati, Haider S. Kadhim, Atheer J. AL-Saffar, Hala S. Arif, Jianmin Fu, and Brian L. Wickes, Haidar A. Shamran, Avan H. Ghaib, Qasim S. Al-Mayah, and Ali H. Bayati, Haider S. Kadhim, Atheer J. AL-Saffar, Hala S. Arif, Jianmin Fu, and Brian L. Wickes, Haidar A. Shamran, Avan H. Ghaib, Qasim S. Al-Mayah, and Ali H. Bayati, | | المستفيدين : | Ministry of Health | | المستخلص : | Among all other viruses, human cytomegalovirus (HCMV) is the most frequent cause of congenital infection worldwide. Strain variation in HCMV may predict severity or outcome of congenital HCMV disease. Previous studies have associated a particular genotype with specific sequelae or more severe illness, but the results were contradictory. There are no previous studies addressing the genotype of HCMV in Iraq. Therefore, the present study is aimed at molecular detection and genotyping of HCMV isolated from symptomatic congenitally/perinatally infected neonates. This prospective study comprised 24 serum samples from symptomatic neonates with congenital/perinatal infection. Viral DNA was extracted from these serum samples; nested polymerase chain reaction was used to amplify the HCMV gB (UL55) gene. Polymerase chain reaction products of the second round of amplification were subjected to direct Sanger sequencing. Bioedit and MEGA5 software (EMBL-EBI, Hinxton, Cambridgeshire, UK) were used for alignment and construction of a phylogenetic tree. Humancytomegalovirus DNA was detected in 23 of 24 samples (95.8%). According to the phylogenetic analysis, three genotypes of the virus were identified; gB1, gB2, and gB3 genotypes. However, the gB4 genotype was not detected. Human cytomegalovirus gB3 was the most frequent genotype: 14 of 24 (58.33%) among symptomatic infected infants, followed by gB1 (6/24; 25%) and gB2 (4/24; 16.67%). A mixed HCMV infection with gB3/ gB1 was detected in only one case. Human cytomegalovirus gB3 was the most predominant genotype among symptomatic congenitally/ perinatally HCMV-infected neonates. No association was found between B3 genotype and specific clinical presentation. Jaundice was the most common clinical feature among symptomatically infected neonates, followed by hepatosplenomegaly. | | المستخلص باللغة العربية : | None | | كلمات مفتاحية : | Genotyping, Cytomegalovirus, Neonates, Symptomatic, Iraq | | الرابط : | doi:10.4269/ajtmh.18-0152 |
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